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产品说明/Specification Sheet
产品名称: |
Con A Type Ⅳ 刀豆蛋白A Ⅳ |
产品编号: |
C2010-25MG |
规格: |
25mg |
价格: |
¥160 |
产品类别: |
生化试剂 |
CAS#: |
11028-71-0 |
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品牌: |
sigma |
级别: |
蛋白质 |
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分子式: |
C15H24 |
分子量: |
0 |
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危险品: |
NO |
危险品系数: |
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储存条件: |
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说明书: |
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产品属性:
Concanavalin A from Canavalia ensiformis (Jack bean)
Type IV, lyophilized powder
Description
Analysis Note |
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C. |
Biochem/physiol Actions |
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6. |
Preparation Note |
Produced from Type III (C2631) using acetic acid by the method of Olson and Liener.1 |
Quality |
Highly purified |
Properties
type |
Type IV |
form |
lyophilized powder |
potency |
<64 μg per mL agglutination activity |
total impurities |
Carbohydrate, essentially free |
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salt, essentially free |
storage temp. |
−20°C |
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