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产品说明/Specification Sheet
产品名称: |
CollagenaseⅠ 胶原酶Ⅰ |
产品编号: |
C0130-100mg |
规格: |
100mg |
价格: |
¥350 |
产品类别: |
生化试剂 |
CAS#: |
9001-12-1 |
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品牌: |
Sigma |
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危险品: |
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说明书: |
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产品属性:
Description
Frequently Asked Questions |
Live Chat and Frequently Asked Questions are available for this Product. |
Other Notes |
View more information on collagenase and cell dissociation at www.sigma-aldrich.com/enzymeexplorer. |
Preparation Note |
Equivalent to first 40% ammonium sulfate fraction of Mandl, I., et al., J. Clin. Invest., 32, 1323 (1953). |
Quality |
Also contains clostripain, nonspecific neutral protease, and tryptic activities. |
Unit Definition |
One collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.
One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.
One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.
One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT. |
Properties
form |
essentially salt-free, lyophilized powder |
storage temp. |
−20°C |
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